Skip to main content

Table 2 Effects of QTL genotype on placental and embryonic gene expression and weight, birth weight and litter size

From: Altered placental expression of PAPPA2 does not affect birth weight in mice

  C57BL/6 DBA/2 P-value
Placental gene expression N = 9 N = 17  
   PAPPA2 mRNA 2.8 ± 0.2 1.1 ± 0.2 < 0.0001
   PAPPA2 proteina 1.5 ± 0.2 0.6 ± 0.2 0.003
   PAPPA mRNA 2.3 ± 0.6 1.8 ± 0.5 0.46
   IGFBP-5 mRNA 1.5 ± 0.4 1.6 ± 0.3 0.78
Embryonic gene expression N = 9 N = 17  
   Embryonic PAPPA2 mRNA 0.8 ± 0.1 0.8 ± 0.1 0.69
Phenotypic outcomes N = 10 N = 16  
   Placental mass at E12.5 (g) 0.084 ± 0.007 0.087 ± 0.005 0.72
   Embryonic mass at E12.5 (g) 0.091 ± 0.004 0.083 ± 0.003 0.16
   Number of embryos at E12.5 3.8 ± 0.5 4.1 ± 0.4 0.71
   Number of spontaneous abortions at E12.5 1.5 ± 0.3 0.9 ± 0.3 0.21
   Birth weight (g)b 1.38 ± 0.03 1.31 ± 0.04 0.32
   Litter sizeb 3.2 ± 0.5 3.8 ± 0.7 0.55
  1. mRNA and protein levels are expressed relative to reference samples run in each assay
  2. Values are least squares means ± standard error
  3. aSample sizes were reduced for PAPPA2 protein measurements (to 9 C57BL/6 and 11 DBA/2) because some females carried only one embryo and placenta, which were used for mRNA quantification
  4. bBirth weight and litter size were measured in 58 C57BL/6 genotype pups from 13 females and in 30 DBA/2 pups from 8 females