Table 1 Primer and probe sequences used in qRT-PCR
From: Altered placental expression of PAPPA2 does not affect birth weight in mice
Gene | Forward Primer | Reverse Primer | Probe (contains fluorophore, 6-FAMâ„¢, on the 5' end and quencher, BHQ-1â„¢, at the 3' end) |
|---|---|---|---|
PAPPA2 | GGGACAAGGAAGCTCTCAGT | CAGGGATCATCACAGGATTC | CATGCTTGGCCACACCAACATCATGATCCA |
IGFBP-5 | AAAGAGCTACGGCGAGCAAA | AGTAGGTCTCTTCAGCCATCTC | AGACTCTCGGGAACACGAGGAACCCA |
PAPPA | CACAATGGACTCTGTGATGCT | TCTCCCTTCTAGGCAAAGGT | TGGTTCCCACCCATCGATGG |
β-actin | CGTGAAAAGATGACCCAGAT | GGTACGACCAGAGGCATACA | ACCTTCAACACCCCAGCCATGT |