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Table 4 Cleavage rate and development of oocytes fertilized by In Vitro fertilization and ICSI with non-treated, scrotal heat stressed and γ-radiated spermatozoa

From: Selection against spermatozoa with fragmented DNA after postovulatory mating depends on the type of damage

Technique Sperm treatment Total oocytes (experiments) 2-cell embryos (%)   Blastocyst (%)
IVF Control 107 (5) 99 (93)a   95 (96)a
  Scrotal Heat Stress 223 (8) 29 (13)b   26 (90)a
  γ-Radiation 85 (5) 56 (66)c   36 (63)b
  Sperm treatment Injected Oocytes (experiments) Surviving Oocytes (%) 2-cell embryos
(%)
Blastocyst
(%)
ICSI Control 72 (3) 53 (74) 46 (87)a 31 (67)a
  Scrotal Heat Stress 232 (6) 158 (68) 74 (47)b 4 (6)b
  γ-Radiation 131 (4) 94 (72) 65 (69)c 28 (43)c
  1. Means ± SEM. Different alphabetical superscript indicates significant differences (P < 0.05, ANOVA analysis) among treatments.