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Table 3 Effect of scrotal heat stress and γ-radiation on Tail length on Comets from spermatozoa recovered after in vitro fertilization from zona pellucida and assessed by neutral Comet assay

From: Selection against spermatozoa with fragmented DNA after postovulatory mating depends on the type of damage

Treatment

Tail Length

Tail Moment

 

IVF

ZP†

IVF

ZP†

Control

16.20 ± 1.02aα

14.76 ± 3.98a

4.55 ± 0.56aα

3.72 ± 1.30a

(n = 9)

    

Scrotal Heat Stress

26.64 ± 3.34aβ

15.96 ± 2.92b

14.82 ± 2.34aβ

8.96 ± 3.31b

(n = 9)

    

γ-Radiation

46.95 ± 6.55aγ

21.43 ± 5.84b

29.90 ± 6.34aγ

8.68 ± 3.15b

(n = 8)

    
  1. Significant differences in tail length and tail moment from spermatozoa recovered in each step of IVF, within a treatment, were denoted with different Latin alphabetical superscript in each row; and within an IVF step were denoted with different Greek superscripts in each column. Means ± SEM. Values with different superscripts differ significantly (P < 0.05) Ep, epididymal sperm; IVF, sperm population recovered from in vitro fertilization drop; ZP, sperm population attached to Zona Pellucida. † Indicates no significant differences among treatments within ZP.
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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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