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Table 3 Effect of scrotal heat stress and γ-radiation on Tail length on Comets from spermatozoa recovered after in vitro fertilization from zona pellucida and assessed by neutral Comet assay

From: Selection against spermatozoa with fragmented DNA after postovulatory mating depends on the type of damage

Treatment Tail Length Tail Moment
  IVF ZP† IVF ZP†
Control 16.20 ± 1.02 14.76 ± 3.98a 4.55 ± 0.56 3.72 ± 1.30a
(n = 9)     
Scrotal Heat Stress 26.64 ± 3.34 15.96 ± 2.92b 14.82 ± 2.34 8.96 ± 3.31b
(n = 9)     
γ-Radiation 46.95 ± 6.55 21.43 ± 5.84b 29.90 ± 6.34 8.68 ± 3.15b
(n = 8)     
  1. Significant differences in tail length and tail moment from spermatozoa recovered in each step of IVF, within a treatment, were denoted with different Latin alphabetical superscript in each row; and within an IVF step were denoted with different Greek superscripts in each column. Means ± SEM. Values with different superscripts differ significantly (P < 0.05) Ep, epididymal sperm; IVF, sperm population recovered from in vitro fertilization drop; ZP, sperm population attached to Zona Pellucida. † Indicates no significant differences among treatments within ZP.