Figure 7
Proliferative response and inhibition of hCG-induced CREB phosphorylation by P2Y agonists in mouse theca cells. Theca cells were stimulated for 48 h with UTP, UDP, or ATP using the indicated concentrations (log M); controls corresponded to cells incubated in media supplemented with 0.1% serum (basal) or with 10% FBS (S). Cell proliferation was evaluated by [3H]-thymidine incorporation, data are the mean ± S.E.M. of 4 independent determinations. B) Time-course of CREB phosphorylation in TIC induced by 2 IU hCG; the 43-kDa band was quantified as in Figure 1B showing that maximal effect was reached in 10-15 min of incubation. C) CREB-phosphorylation elicited by 2 IU hCG in TIC incubated for 10 min in basal conditions and in the presence of 0, 10, or 100 μM UTP; the 43-kDa band was quantified as in B), data are from 3 independent experiments in triplicate. (*p < 0.05 vs. basal, one-way ANOVA and Bonferroni's test).