Effect of estrogens on boar sperm capacitation in vitro

Table 2 Number of capacitated sperm in control and experimental samples after 120 of capacitation

Group	Control	1 nM	10 nM	100 nM	1 μM	10 μM	100 μM
E1	57.4 ± 1.14	57.5 ± 1.89	57.55 ± 1.78	57.37 ± 2.62	58.11 ± 1.58	60.12 ± 1.12**	61.25 ± 1.56***
E2	57.4 ± 1.14	58.00 ± 1.61	58.80 ± 1.62	58.11 ± 2.11	58.40 ± 0.89	60.10 ± 1.22**	61.81 ± 1.22***
E3	57.4 ± 1.14	56.89 ± 1.75	56.14 ± 1.35	57.00 ± 1.76	57.78 ± 2.45	60.22 ± 1.56**	61.54 ± 1.67***
EE2	57.4 ± 1.14	57.84 ± 2.09	57.70 ± 1.93	58.42 ± 1.36	58.30 ± 1.98	60.10 ± 2.31**	61.43 ± 1.37***

Sperm samples from boar E (with no significant responsiveness to E2 at concentration of 1 μM) were capacitated in the presence of six different concentrations (1 nM - 100 μM) of four estrogens and analyzed by CTC and immunocytochemistry with ACR.2. In each group, at least 5 samples were analyzed. In CTC assay and immunocytochemistry with ACR.2 antibody, only highly correlated results (difference < 5%) were used in the subsequent statistical analysis. All estrogens significantly accelerated the capacitation progress at 10 μM concentration. Differences were analyzed by KW-ANOVA; post hoc comparison was performed by multiple comparisons of mean ranks. *P < 0.05, **P < 0.01, ***P < 0.001.

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