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Table 1 Number of capacitated sperm in control and experimental samples after 120 of capacitation

From: Effect of estrogens on boar sperm capacitation in vitro

Group

Control

1 nM

5 nM

10 nM

100 nM

1 μM

10 μM

E1

55.00 ± 1.56

55.5 ± 0.93

55.45 ± 1.79

56.25 ± 1.28

57.60 ± 1.26**

59.75 ± 1.58***

61.50 ± 0.93***

E2

55.00 ± 1.56

56.00 ± 1.41

56.74 ± 1.21*

56.80 ± 1.62*

57.73 ± 1.27***

60.20 ± 1.93***

62.00 ± 1.41***

E3

55.00 ± 1.56

54.88 ± 1.36

55.14 ± 1.75

56.14 ± 1.35

57.00 ± 0.89**

59.78 ± 1.48***

61.71 ± 0.76***

EE2

55.00 ± 1.56

55.44 ± 1.81

54.99 ± 2.25

55.80 ± 1.93

56.82 ± 2.04*

58.30 ± 2.91**

62.00 ± 1.41***

  1. Sperm samples from boar A (with high responsiveness to E2) were capacitated in the presence of six different concentrations (1 nM - 10 μM) of four estrogens and analyzed by CTC and immunocytochemistry with ACR.2. In each group, at least 5 samples were analyzed. In CTC assay and immunocytochemistry with ACR.2 antibody, only highly correlated results (difference < 5%) were used in the subsequent statistical analysis. All estrogens significantly accelerated the capacitation progress in a concentration-dependent manner. E2 had the first significant effect at 10 nM concentration, other three estrogens at 100 nM concentration. Differences were analyzed by KW-ANOVA; post hoc comparison was performed by multiple comparisons of mean ranks. *P < 0.05, **P < 0.01, ***P < 0.001.