Figure 4

Activated phospho-STAT1 in BRCA1⁺ovarian epithelial cells. (A) Wild type HOSE-E6E7 and OVCA429 cancer cells and BRCA1-mutant at-risk (HOSE-642, HOSE-636) cells and BRCA1-mutant ovarian cancer cells (UWB1) were subjected to PAF treatment for 20 minutes and phospho-STAT1 (Y701) detection by western blot. (B) Summary of intensity of SATA1 and phosphor-STAT1 induced by PAF treatment in HOSE-642, UWB1 and HOSE-636 cells. Phosphor-STAT1 was significantly (p < 0.01) increased in all BRCA1-mutant cells. (C) Activation of phosphor-STAT1 at different time points (0, 5, 20 60 min and 360 min) of PAF treatment (100 nM) in BRCA1-mutant HOSE-642 and UWB1 cells. (D) Equal amount of protein lysate of the cells of untreated, treated with PAF (100 nM) alone or in combination of PAF and PAFR-inhibitor (10 μM) and GB (100 μM) were applied in western blot detection. DMSO and pure IgG (not shown) were used as control treatment for 20 minutes in both HOSE-642 and UWB1 cells. Equal loading of protein was calibrated with expression of β-actin.