Figure 1

Molecular characteristics of the BRCA1 mutation in (BRCA1⁺) ovarian epithelial cells. (A) DNA extracted from the HOSE-636 and HOSE-E6E7 cells were subjected to BRCA1 gene sequence analysis with the standard protocol. 1961 delA mutation was detected in HOSE-636 cells. (B) Total protein lysis (30 μg) of Western blot showed pieces of mutant fragments of BRCA1 protein in HOSE-E6E7, HOSE-636 HOSE-642, and UWB1 cells were applied on SDS-PAGE and western blot analysis. Intact BRCA1 protein and its fragments were revealed by BRCA1 antibody. The separated western blots are required because of the nature and working conditions are different between BRCA1 intact protein and the truncated peptides. (C) PAFR protein was detected by western blot in wild type HOSE-E6E7 and BRCA1-mutant HOSE-636, HOSE-642, and UWB1 cell lines with a specific polyclonal antibody against human PAFR. The equal protein loading was normalized by β-actin.