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Table 2 Common and Gene names, and main known functions of the protein signals detected by ESI mass spectrometry

From: Characterization of a diverse secretome generated by the mouse preimplantation embryo in vitro

Protein name

Common Name

Gene Name

Main Function, where known

Essential for preimplantation development

   

NLR family, pyrin domain containing 5

Mater

Nlrp5

Cell surface expression protein essential for normal early embryo development

Peptidyl arginine deiminase, type VI

ePAD

Padi6

Catalyzes the deimination of arginine residues of proteins. May be involved in cytoskeletal reorganization in the egg and early embryo.

Modification of proteins

   

Peptidylprolyl isomerase A

cyclophilin A

Ppia

Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides, accelerates the folding of proteins.

Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta/delta polypeptide

14-3-3 zeta

Ywhaz

Adapter protein that binds to a large number of partners by recognition of a phosphoserine or phosphothreonine motif. Generally results in the modulation of the activity of the binding partner.

Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide

14-3-3 gamma

Ywhag

As above.

REDOX regulation

   

Lactate dehydrogenase B

LHD-2

Ldhb

Catalyzes reaction: (S)-lactate + NAD(+) = pyruvate + NADH.

Prolyl 4-hydroxylase, beta polypeptide (ERp59)

Protein disulfide-isomerase

P4hb

Catalyzes the formation, breakage and rearrangement of disulfide bonds. Present at high levels in many cells and actively shed by many cells.

Protein disulfide-isomerase A3 (ERp61)

Protein disulfide-isomerase

Pdia3

Catalyzes the formation, breakage and rearrangement of disulfide bonds

Peroxiredoxin 1

Thioredoxin peroxidase 2

Prdx1

Catalyzes reactions: 2 R'-SH + ROOH = R'-S-S-R' + H(2)O + ROH.

Chaperonins

   

Calreticulin

Calregulin

Calr

Calcium binding chaperonin, promotes protein folding. Can act as a cell surface lectin.

Heat shock protein HSP 90-alpha

HSP 90

Hsp90aa1

A stress actvated chaperonin with ATPase activity. Many cellular functions and targets.

2'-5' oligoadenylate synthetase 1C

 

Oas1c

Interferon activated anti-viral agent

Miscellaneous or Ill-defined function

   

Spindlin isoform 1

30000 metaphase complex

Spin1

A meiotic spindle binding protein present in oocytes.

Glycogenin 1

 

Gyg1

Self-glucosylates forming oligosaccharide primer for glycogen synthase

Transducin-like enhancer of split 6

Groucho-related protein 6

Tle6

Transcriptional co-repressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling.

2,3-bisphosphoglycerate mutase

2,3-bisphosphoglycerate synthase

Bpgm

Regulates hemoglobin oxygen affinity. Catalyzes reaction: 3-phospho-D-glyceroyl phosphate = 2,3-bisphospho-D-glycerate.

Phosphatidylethanolamine binding protein

HCNPpp

Pebp1

Binds ATP, opioids and phosphatidylethanolamine. Serine protease inhibitor, may be involved in the function of the pre-synaptic cholinergic neurons of the central nervous system.

Zinc finger, BED domain containing 3

 

Zbed3

Still to be defined, transcripts detected in 2-cell embryo library

F-box domain containing protein

  

F-box domain is approximately 50 amino acids long and is found in the N-terminal half of a variety of proteins commonly associated with the leucine rich repeats

Oviductal glycoprotein 1

OGP

Ovgp1

Belongs to the glycosyl hydrolase 18 family. Released by oviduct, binds to zona pelleucida.