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Table 2 Common and Gene names, and main known functions of the protein signals detected by ESI mass spectrometry

From: Characterization of a diverse secretome generated by the mouse preimplantation embryo in vitro

Protein name Common Name Gene Name Main Function, where known
Essential for preimplantation development    
NLR family, pyrin domain containing 5 Mater Nlrp5 Cell surface expression protein essential for normal early embryo development
Peptidyl arginine deiminase, type VI ePAD Padi6 Catalyzes the deimination of arginine residues of proteins. May be involved in cytoskeletal reorganization in the egg and early embryo.
Modification of proteins    
Peptidylprolyl isomerase A cyclophilin A Ppia Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides, accelerates the folding of proteins.
Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta/delta polypeptide 14-3-3 zeta Ywhaz Adapter protein that binds to a large number of partners by recognition of a phosphoserine or phosphothreonine motif. Generally results in the modulation of the activity of the binding partner.
Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide 14-3-3 gamma Ywhag As above.
REDOX regulation    
Lactate dehydrogenase B LHD-2 Ldhb Catalyzes reaction: (S)-lactate + NAD(+) = pyruvate + NADH.
Prolyl 4-hydroxylase, beta polypeptide (ERp59) Protein disulfide-isomerase P4hb Catalyzes the formation, breakage and rearrangement of disulfide bonds. Present at high levels in many cells and actively shed by many cells.
Protein disulfide-isomerase A3 (ERp61) Protein disulfide-isomerase Pdia3 Catalyzes the formation, breakage and rearrangement of disulfide bonds
Peroxiredoxin 1 Thioredoxin peroxidase 2 Prdx1 Catalyzes reactions: 2 R'-SH + ROOH = R'-S-S-R' + H(2)O + ROH.
Chaperonins    
Calreticulin Calregulin Calr Calcium binding chaperonin, promotes protein folding. Can act as a cell surface lectin.
Heat shock protein HSP 90-alpha HSP 90 Hsp90aa1 A stress actvated chaperonin with ATPase activity. Many cellular functions and targets.
2'-5' oligoadenylate synthetase 1C   Oas1c Interferon activated anti-viral agent
Miscellaneous or Ill-defined function    
Spindlin isoform 1 30000 metaphase complex Spin1 A meiotic spindle binding protein present in oocytes.
Glycogenin 1   Gyg1 Self-glucosylates forming oligosaccharide primer for glycogen synthase
Transducin-like enhancer of split 6 Groucho-related protein 6 Tle6 Transcriptional co-repressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling.
2,3-bisphosphoglycerate mutase 2,3-bisphosphoglycerate synthase Bpgm Regulates hemoglobin oxygen affinity. Catalyzes reaction: 3-phospho-D-glyceroyl phosphate = 2,3-bisphospho-D-glycerate.
Phosphatidylethanolamine binding protein HCNPpp Pebp1 Binds ATP, opioids and phosphatidylethanolamine. Serine protease inhibitor, may be involved in the function of the pre-synaptic cholinergic neurons of the central nervous system.
Zinc finger, BED domain containing 3   Zbed3 Still to be defined, transcripts detected in 2-cell embryo library
F-box domain containing protein    F-box domain is approximately 50 amino acids long and is found in the N-terminal half of a variety of proteins commonly associated with the leucine rich repeats
Oviductal glycoprotein 1 OGP Ovgp1 Belongs to the glycosyl hydrolase 18 family. Released by oviduct, binds to zona pelleucida.