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Table 1 Protein signals detected by ESI mass spectrometry in eight B6 or F1 embryo-conditioned media

From: Characterization of a diverse secretome generated by the mouse preimplantation embryo in vitro

Protein name

Mr (Da)

Average score

Gi #

Positive cultures

Strain distribution B6:F1 (M) *

Lactate dehydrogenase B

36,549

358 ± 48

6678674

8

3:4 (1)

Peptidyl arginine deiminase, Type VI

76,855

375 ± 84

23346537

7

3:3 (1)

Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta/delta polypeptide

27,839

117 ± 24

112696

7

3:4 (0)

Calreticulin

47,965

115 ± 20

6680836

7

3:3 (1)

Prolyl 4-hydroxylase, beta polypeptide (ERp59)

57,108

116 ± 16

129729

5

2:3 (0)

NLR family, pyrin domain containing 5

125,421

169 ± 48

7106379

4

2:2 (0)

Oviductal glycoprotein 1

78,758

180 ± 53

33468849

4

2:2 (0)

Protein disulfide-isomerase A3 (ERp61)

2,121

73 ± 7

545439

4

2:2 (0)

Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, gamma polypeptide

28,345

94 ± 26

3065929

4

2:2 (0)

Glycogenin 1

37,378

84

7305121

2

1:1 (0)

Zinc finger, BED domain containing 3

25,293

114

21312432

2

1:1 (0)

Transducin-like enhancer of split 6

65,074

77

16716583

2

1:1 (0)

2,3-bisphosphoglycerate mutase

29,960

91

6680806

2

1:1 (0)

Peroxiredoxin 1

22,162

150

6754976

1

1:0 (0)

Phosphatidylethanolamine binding protein

20,847

127

1517864

1

1:0 (0)

F-box domain containing protein

54,417

127

28892973

1

1:0 (0)

Heat shock protein HSP 90-alpha

84,735

114

6754254

1

1:0 (0)

Spindlin isoform 1

27,119

60

6755620

1

0:1 (0)

Peptidylprolyl isomerase A

17,960

54

6679439

1

0:1 (0)

2'-5' oligoadenylate synthetase 1C

42,884

53

15809044

1

1:0 (0)

   

# GI number of the protein database record at NCBI

 

* Distribution of positive cultures - M is mixed of B6 and F1