Figure 3

Western blotting detection of PCNA protein in the ovaries. (A), The expressions of. PCNA protein by western blot; (B), Semiquantitative analysis of the western-blot result. (1), Control (ovary of 2-day-old rat); (2), Ovary of 2-day-old rat cultured for 4 days; (3), Ovary of 2-day-old rat cultured for 8 days; (4), Ovary of 2-day-old rat cultured for 4 days with 50 ng/ml EGF; (5), Ovary of 2-day-old rat cultured for 8 days with 50 ng/ml EGF. Data are presented as means ± SEM (n = 3). **, P < 0.01 vs control group; Δ, P < 0.05 vs group 3.