Figure 2

RT-PCR detection of c-erbB ₂ mRNA in the ovaries. (A), The expressions of c-erbB ₂ mRNA (322 bp) by RT-PCR; (B), Semiquantitative analysis of the RT-PCR result; (1), Control (ovary of 2-day-old rat); (2), Ovary of 2-day-old rat cultured for 4 days; (3), Ovary of 2-day-old rat cultured for 8 days; (4), Ovary of 2-day-old rat cultured for 4 days with 50 ng/ml EGF; (5), Ovary of 2-day-old rat cultured for 8 days with 50 ng/ml EGF. Data are presented as means ± SEM (n = 3). *, P < 0.05; **, P < 0.01 vs control group; Δ, P < 0.05 vs group 3.