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Figure 2 | Reproductive Biology and Endocrinology

Figure 2

From: Homeobox A7 increases cell proliferation by up-regulation of epidermal growth factor receptor expression in human granulosa cells

Figure 2

HOXA7 regulates granulosa cell proliferation. KGN cells were transiently transfected with scrambled siRNA or HOXA7 siRNA and the effect of HOXA7 knockdown was verified at the mRNA level by real-time PCR (A) and protein level by Western blotting (B). (C) After 36 h of transfection, KGN cells were reseeded into a 96-well plate and the cell viability was detected by MTT assay. SVOG cells were transiently transfected with control vector or HOXA7 plasmid and the effect of HOXA7 overexpression was verified at the mRNA level by real-time PCR (D) and protein level by Western blotting (E). (F) After 36 h of transfection, SVOG cells were reseeded into a 96-well plate and the cell viability was detected by MTT assay. The data derived from at least three separate sets of experiments were standardized to the corresponding control. a, P < 0.05 compared with control siRNA or control vector.

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