HOXA7 and EGFR expression in human granulosa cells. Expression of HOXA7 and EGFR was detected in primary hGCs, the immortalized human granulosa cell line, SVOG, and the granulosa tumor cell line, KGN, cells by both real-time PCR and Western blotting. (A) Real-time PCR results of relative HOXA7 mRNA expression. (B) The representative autoradiographs of HOXA7 detected by Western blotting (indicated by arrow). (C) Real-time PCR results of relative EGFR mRNA expression. (D) The representative autoradiographs of EGFR detected by Western blotting. The data derived from at least three separate sets of experiments were standardized to the corresponding control, and the statistical results are presented in the column graphs. a, P < 0.05 compared with hGC; b, P < 0.05 compared with SVOG.