Table 2 Oligonucleotide primers used for Real-time RT-PCR analysis
Gene | Primer | Sequence | Position |
|---|---|---|---|
EMMPRIN | Forward | CGGAGTATGAGGTGGACTCAGAA | 265-288 |
(NM_001075371) | Reverse | GCTCCGGAAGGAAGATGCA | 327-308 |
MMP-1 | Forward | TGGACCAGCAATTTCCAAGAT | 618-639 |
(NM_174112) | Reverse | TCCAAGGGAATGGCCAAA | 686-668 |
MMP-2 | Forward | GGAGAGGCTGACATCATGATCA | 677-699 |
(NM_174745) | Reverse | CCCGTCTTTGCCATCAAAA | 751-770 |
MMP-9 | Forward | CATTTCTTCAAGGCTGGGAAGTA | 1615-1638 |
(NM_174174) | Reverse | GCGCAGGCCACTTGCT | 1708-1692 |
MMP-14 | Forward | TGCCGAGCCTTGGACTGT | 704-722 |
(NM_174390) GAPDH (NM_001034034) | Reverse Forward Reverse | GCCACCAGAAAGATGTCATTCC AAGGCCATCACCATCTTCCA CCACCACATACTCAGCACCAGCAT | 763-741 280-300 355-331 |