Figure 1
From: Identification of calcium-binding proteins associated with the human sperm plasma membrane
2D autoradiogram of radioiodinated acidic and neutral human sperm surface proteins. The positions of 80K-H and SAP are indicated by arrows, as well as those of previously reported surface proteins; oxygen regulated protein 150 (HYOU1), angiotensin converting enzyme (ACE), HSP86 (HSPC1), Bip (HSPA5), sperm-specific surface hyaluronidase (PH-20) and HSP70-1 (HSPA2). The positions of four intracellular proteins; valosine containing protein (VCP), calreticulin (CRT), tubulin and actin are indicated by rectangular boxes. Additional non-labelled tubulin isoforms were detected by mass spectrometry to the right of the boxed area. See also Figure 3A and Additional file 1 - Supplementary Figure 1.