Figure 3

A representative western blot of ghrelin (sc anti-goat antibody) protein expression in human myometrium. NP (n = 4), PNL (n = 4) and PL (n = 5) (i) prepro-ghrelin and (ii) pro-ghrelin and (iii) mature ghrelin bands are indicated. The corresponding β-Actin (ACTB) western blot is presented underneath each blot. Molecular weights are indicated in kDa. Quantitative densitometric analysis of each ghrelin isoform is presented below the relevant blot(s) (i-ii), with β-Actin-normalised densitometric units for each protein plotted against pregnancy state ± SEM (indicated with error bars). * indicates a significance value of P < 0.05. Significance values indicated are compared to NP tissue expression.