Figure 2

A representative western blot of ghrelin (sc anti-rabbit antibody) protein expression in human myometrium. NP (n = 4), PNL (n = 4), PL (n = 5) and HeLa cells (H). (i) Prepro-ghrelin and (ii) pro-ghrelin and (iii) mature ghrelin bands are indicated. The corresponding β-Actin (ACTB) western blot is presented underneath each blot. Molecular weights are indicated in kDa. Quantitative densitometric analysis of each ghrelin isoform is presented below the relevant blot(s) (i-iii), with β-Actin normalised densitometric units for each protein plotted against pregnancy state ± SEM (indicated with error bars). * indicates a significance value of P < 0.05. Significance values indicated are compared to NP tissue expression.