Figure 5

Regulatory effects of GnRH I and GnRH II on MMP-26 expression in primary cultured CTB cells. A, The expression of MMP-26 mRNA in CTB cells cultured with GnRH I (gray) or GnRH II (black) at concentrations of 0.1, 1, 10 and 100 nM for 24 h. B, Following 30-minute pretreatment with PD98059 (10 μM, ERK1/2 inhibitor) or SP600125 (10 μM, JNK inhibitor), CTB cells were incubated with GnRH I (gray) or GnRH II (black) at concentrations of 100 nM for 24 h and the mRNA levels of MMP-26 analyzed by Real-time PCR. Levels of mRNA for MMP-26 in each sample were normalized to the corresponding GAPDH. The results derived from both these analyses as well as those from at least three other sets of experiments were standardized to the untreated control and are represented (mean ± SEM; n ≥ 3) in the bar graphs (*, P < 0.05 vs. untreated control).