Figure 3

Different dosage effects of GnRH I and GnRH II on MMP-26 expression in B6Tert-1 cells. A and B, The expression of MMP-26 mRNA (A) and protein (B) in B6Ter-1 cells cultured with increasing concentrations of GnRH I (0, 1, 10, 100 nM or 1 μM) for 24 h. C and D, The expression of MMP-26 mRNA (C) and protein (D) in B6Ter-1 cells cultured with increasing concentrations of GnRH II (0, 1, 10, 100 nM or 1 μM) for 24 h. Levels of mRNA for MMP-26 in each sample were normalized to the corresponding GAPDH. Levels of protein for MMP-26 were normalized to the corresponding β-actin. The results derived from both these analyses as well as those from at least three other sets of experiments were standardized to the untreated control and are represented (mean ± SEM; n ≥ 3) in the bar graphs (*, P < 0.05 vs. untreated control).