The characteristics of B6Tert-1 cells. A, Western blotting analyses show the expression of GnRH receptor (GnRHR) in JEG-3 and B6Tert-1 cells. Mouse pituitary gonadotrope αT3-1 cells were used as the positive control. B, The B6Tert-1 cells were cultured in the presence of 100 nM GnRH I or GnRH II for 24 h, and their invasive capacities were analyzed by invasion assay. C, The expression of MMP-26 protein in JEG-3, B6Tert-1, primary cultured cytotrophoblast (CTB) cells. The data derived from at least three independent sets of experiments (mean ± SEM; n ≥ 3), and the statistic results are presented in the column graphs (*, P < 0.05 vs. control).