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Table 4 The rate of early stage of apoptosis in oocytes at 3 and 8 hours after siRNA adneovirus infection, was evaluated by Annexin-V staining in different groups (Control, shHsp27(1), shHsp27(2)).

From: Downregulation of both gene expression and activity of Hsp27 improved maturation of mouse oocyte in vitro

 

3 h

8 h

Treatment

Total

No. of early apoptosis oocytes (%)

Total

No. of early apoptosis oocytes (%)

control

130

44 (33.8)

129

44 (34.1)

shHsp27(1)

131

51 (38.9)

131

61 (46.5)*

shHsp27(2)

149

54 (36.2)

125

57 (45.6)*

  1. 3 h and 8 h indicated the time of culture in vitro after adenovirus infection.
  2. *Significant changes within group (* P < 0.05 vs. control).