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Table 1 Primer sequences used for quantitative real-time PCR reactions.

From: Downregulation of both gene expression and activity of Hsp27 improved maturation of mouse oocyte in vitro

Primer name

Genbank accession number

Primer sequence

Location

Product size (bp)

Hsp27

NM_013560

F: 5'-GCCGCACCAGCCTTCAGC-3'

357-374

147

  

R: 5'-CACGCCTTCCTTGGTCTTCACT-3'

482-503

 

Caspase 3

NM_009810

F: 5'-ATGGGAGCAAGTCAGTGGAC-3'

128-147

137

  

R: 5'-CGTACCAGAGCGAGATGACA-3'

245-264

 

Caspase 8

NM_001080126

F: 5'-TGAAGGACAGAAAAGGAACAGA-3'

996-1017

191

  

R: 5'-CTTGTCACCGTGGGATAGGATA-3'

1165-1186

 

Caspase 9

NM_015733

F: 5'-GGCGGAGCTCATGATGTCTGTG-3'

757-778

269

  

R: 5'-TTCCGGTGTGCCATCTCCATCA-3'

1004-1025

 

Cytochrome c

XR_034017

F: 5'-GGAGGCAAGCATAAGACTGG-3'

219-239

214

  

R: 5'-GTCTGCCCTTTCTCCCTTCT-3'

413-432

 

Bmp15

NM_009757

F: 5'-CTGACGACCCTACATTGCCCT-3'

467-487

232

  

R: 5'-TGTACATGCCAGGAACCTCTGG-3'

677-698

 

Gdf9

NM_008110

F: 5'-TCCCAAACCCAGCAGAAGTC-3'

426-445

195

  

R:5'-GGAGGAGGAAGAGGCAGAGTTG-3'

609-620

 

GAPDH

NM_008084

F: 5'-AGGTTGTCTCCTGCGACTTCA-3'

843-903

216

  

R: 5'-GGGTGGTCCAGGGTTTCTTACT-3'

1048-1068

 

beta-actin

NM_007393

F:5'-GAGACCTTCAACACCCCAGC-3'

452-471

263

  

R:5'-ATGTCACGCACGATTTCCC-3'

696-714

 
  1. F, forward; R, reverse