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Figure 4 | Reproductive Biology and Endocrinology

Figure 4

From: Downregulation of both gene expression and activity of Hsp27 improved maturation of mouse oocyte in vitro

Figure 4

Effect of Hsp27 downregulation in GV oocytes on the expression of some key apoptotic proteins and oocyte-derived growth factors. (A) Real time RT-PCR analysis in mouse oocytes after microinjecting Hsp27 antibody. Oocytes were cultured for 18-24 h. (B) Real time RT-PCR analysis in oocytes after infection with Ad-shHsp27. (C-F) Western blot analysis of key apoptotic factors in oocytes after infection with Ad-shHsp27. Measurements were plotted as the mean of at least three biological replicates ± SD. Changes are labeled as significant (*) if P < 0.05.

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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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