Figure 1

Llama seminal plasma protein separation on SDS-PAGE after exposure to different treatments in Experiment 2 (A) and Experiment 3 (B). Seminal plasma samples were reduced, denatured, and separated on 12% polyacrylamide gel and stained with Coomassie Blue R-250. A) Lane 1 was loaded with a molecular mass standard. The remaining lanes were loaded with 20 μg of seminal plasma (SP): Lane 2 - non-treated SP; Lane 3 - SP kept at 38°C for 1 hour; Lane 4 - SP heated to 65°C for 10 min.; Lane 5 - SP heated to 65°C for 1 hour; Lane 6 -- SP treated with charcoal dextran at 4°C for 12 hours; Lane 7 -- SP treated with proteinase K (500 μg/ml) at 38°C for 1 hour; Lane 8 -- phosphate buffered saline (PBS) plus proteinase K (500 μg/ml) at 38°C for 1 hour; * Protein band represents proteinase K. B) Lane 1 was loaded with a molecular mass standard. The remaining lanes were loaded with 30 μg of seminal plasma: Lane 2 - non-treated seminal plasma (SP); Lane 3 - SP kept at 38°C for 12 hours; Lane 4 - phosphate buffered saline (PBS) plus pronase E (500 μg/ml) at 38°C for 1 hour; Lane 5, 6, 7, 8, 9 - Seminal plasma treated with pronase E (500 μg/ml) at 38°C for 1, 3, 6, 9 or 12 hours, respectively. * Protein bands represent pronase E.