Growth of thawed follicles cultured over a 72 hour culture period. (A) Diameter of follicles cultured over 72 hr. Each line represents the follicle diameter measured before and after 72 hr of in vitro culture (N = 50 follicles per group). Data points are average diameters. (B) Increase of follicle diameter after in vitro culture. The increase in the follicle diameter of SSV group was significantly higher than that of the slow-rate freezing group. Columns are mean ± S.D.*P < 0.05 versus the slow-rate freezing group.