Activation of the transcription factor, nuclear factor kappa-B, during the estrous cycle and early pregnancy in the pig

Table 1 Primer and probe sequences used for quantitative RT-PCR analysis

Target^a	Forward/Reverse Primers (5' → 3')^b	Fluorescent Reporter^c	Length of Amplicon^d	GenBank Accession #^e
TNFRSF11A	GCTGACTCTGGAAGAGAAGGTGTT	ATGTGCTGTCCAGACGGTGGTGGTGCCTGT	192 bp	CB475057
	GCCCTGTCCACATATTCGTCTTCTGT
TLR4	ATGGCCTTTCTCTCCTGCCTGA	ATCTGAGAGCTGGGACCCTTGCGTGCAGGT	139 bp	AB188301
	AGGTCCAGTATCTTGACTGATGTGGG
NFKB1	CCCATGTAGACAGCACCACCTATGAT	ACCAGGCTGGCAGCTCTCCTCAAAGCAGCA	132 bp	NM_001048232
	ACAGAGGCTCAAAGTTCTCCACCA
RELA	ACATGGACTTCTCAGCCCTTCTGA	ACACCTGCTCTGCCCAGAGCACTGGGTT	168 bp	CN155798
	CCGAAGACATCACCCAAAGATGCT
NFKBIA	TGTGATCCTGAGCTCCGAGACTTT	TCTACACCTTGCCTGTGAGCAGGGCTGCCT	143 bp	NM_001005150
	TTGTAGTTGGTGGCCTGCAGAATG
NFKBIB	TCATTCTGCAGGTCCAGGTACTCA	TGGATTTCCTCCTGGGCTTTGCTGCTGGCA	89 bp	AK231853
	CACTTGGCGGTGATTCATCAGCAT

^aThe amplification target: TNFRSF11A, Receptor activator of NFKB; TLR4, Toll-like receptor 4; NFKB1; RELA, NFKBIA and NFKBIB, Inhibitors of κB α and β, respectively.
^bThe forward and reverse DNA oligos used in the amplification of the target. Forward and reverse do not necessarily indicate the in vivo direction of transcription.
^cThe DNA oligo sequence of the dual-labeled probe (possessing the FAM reporting dye on the 5' end and the TAMRA quenching dye on the 3' end) used to measure amount of amplified target during each cycle of quantitative RT-PCR.
^dThe length of the amplicon created during PCR.
^eThe accession number to the porcine sequence that was utilized during primer and probe design.

ISSN: 1477-7827