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Figure 4 | Reproductive Biology and Endocrinology

Figure 4

From: Activation of the transcription factor, nuclear factor kappa-B, during the estrous cycle and early pregnancy in the pig

Figure 4

In Situ hybridization analysis of ESR1 mRNA expression in porcine endometrium. Protected transcripts in endometrium from days 0, 5, 7.5, 10, 12, 13, 15 and 17 of the estrous cycle and days 10, 12, 13, 15 and 17 of pregnancy were visualized by liquid emulsion autoradiography and imaged under bright-field and dark-field illumination. Note the greatest expression is prior to (day 17), during (day 0) and after estrus (day 5). ESR1 expression is abundant in the luminal epithelium (LE) and glandular epithelium (GE) during days 0 and 5 while only in the LE on day 17 of the estrous cycle. A representative day 5 section was hybridized with radiolabeled sense cRNA probe to serve as a negative control. All other images are representative from four biological replications. 4× Objective and 10× eyepiece for original magnification 40×.

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