Figure 2

Electron microscopic analysis of the effect of ACH treatment on sperm morphology. A: In the control, there are no mid-piece sperm that are surrounded by cytoplasmic droplets; B: After ACH treatment, there are some mid-piece sperm surrounded by cytoplasmic droplets (black arrows); C: Comparison of the percentage of sperm droplet retention between the control and ACH groups. *P < 0.05 versus the control group.