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Table 2 qPCR validation of microarray results for selected genes

From: Cellular and molecular evidence for a role of tumor necrosis factor alpha in the ovulatory mechanism of trout

Clone ID

Clone name

Regulation

Microarray

qPCR

CA344136

Small inducible cytokine B14 precursor

Up-regulated

0.33

1.33

CU068239

Leukocyte cell-derived chemotaxin 2

Up-regulated

0.61

0.41

CA374135

CCL4

Up-regulated

0.70

2.36

CA351440

TNF decoy receptor

Up-regulated

0.49

0.38

DW550559

Coronin-1C

Down-regulated

-1.18

-0.24

CU067298

Triosephosphate isomerase

Down-regulated

-1.28

-2.47

CU069718

Serine protease-like protein-1

Down-regulated

-0.43

-0.68

CA364711

Inhibitor of apoptosis protein 3

Down-regulated

-0.88

-1.24

  1. Data from real-time PCR (qPCR) is shown as -ΔΔC t (n = 3) and data from microarray as mean log2 ER (expression ratio).