Table 1 Sequences of primers used in gene expression analyses by qPCR
Clone ID | Clone name | Primer sequence (5'-3') | Amplicon size (bp) |
|---|---|---|---|
AJ278085 | Tumor necrosis factor alpha (TNFα) | (F) AGCATGGAAGACCGTCAA (R) TTCGTTTACAGCCAGGCT | 271 |
CA344136 | Small inducible cytokine B14 precursor | (F) AGCTGAAGCCTACAAGTGCAGGTGC (R) TCCTTCCAGATCCGGAACCAC | 219 |
CU068239 | Leukocyte cell-derived chemotaxin 2 | (F) AGGACACCACGGCGCAAGCAGAG (R) TGTCACACATCTGGACGTGGACG | 314 |
CA374135 | CCL4 | (F) TGCTTGTTCTGGTACTCAGTGCCA (R) TGTCTGAGGACCTGGCACACACC | 207 |
CA351440 | TNF decoy receptor | (F) TCTCCTGGTATTTGCGCTCTGTGGT (R) TATAAGTCGGTGTGTGAGCGCCTGA | 65 |
DW550559 | Coronin-1C | (F) AGTGGTGCGAGCGAGTAAGT (R) GACAGGTCCCGTATGACCAC | 241 |
CU067298 | Triosephosphate isomerase | (F) GCTTCCTTGTGGGTGGTGCTTC (R) CCACAGGAAGGAGCAGATGACACC | 181 |
CU069718 | Serine protease-like protein-1 | (F) ACCAAAAACATGCTGTGTGC (R) CCCTCCATTTGAAGTGATCC | 111 |
CA364711 | Inhibitor of apoptosis protein 3 | (F) TGCTTCTGCTGTGGAGGGATG (R) CGTGGTCAATAGGGTGCTGGA | 244 |
AF005026 | Kallikrein (KT-14) | (F) CCAGGAGGTGGAATTAGGATGAGA (R) GCAGAGCAGCAGTCAGGAACA | 167 |
AF223388 | Novel osteopontin-like protein (NOP) | (F) CAGCAGAGAGCAACGAGAGCCAT (R) AGGGTCATAGAGCACCTTCCAGG | 182 |
U67854 | Trout ovulatory protein-2 (TOP-2) | (F) GGGATGTGTGCGGAGTTATGCT (R) ACTGTGTCGGTGCAATGCAGTCAT | 99 |
AF156738 | Trout decoy receptor (TDcR) | (F) GCGTCGGTTAGCAATGAGGC (R) GGTTTCTTGCCTCCCTCTTGTG | 45 |
CA380218 | Macrophage migration inhibitory factor (MIF) | (F) AGGCAACAGATGAACTGGCCAAGG (R) AGGCCACATTCGCTGCCTCCA | 258 |
AF308735 | 18S ribosomal RNA | (F) CGGAGGTTCGAAGACGATCA (R) TCGCTAGTTGGCATCGTTTAT | 62 |