Figure 4From: Cellular and molecular evidence for a role of tumor necrosis factor alpha in the ovulatory mechanism of troutSteroid production of brown trout preovulatory follicles treated with rtTNFα in vitro. Preovulatory trout follicles were incubated in the absence or presence of coho salmon LH (sLH; 25 ng/ml), different concentrations of rtTNFα (1, 10 and 50 ng/ml) (A) or sLH in the absence or presence of 50 ng/ml of rtTNFα (B) for 24 h at 15°C. Testosterone levels were measured in the medium at the end of the incubation period. Each bar represents the mean ± SE of two independent experiments, each with ovarian tissue from a separate female and assayed in triplicate. Statistically significant (p < 0.05) differences among groups are indicated by different letters.Back to article page