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Figure 4 | Reproductive Biology and Endocrinology

Figure 4

From: Cellular and molecular evidence for a role of tumor necrosis factor alpha in the ovulatory mechanism of trout

Figure 4

Steroid production of brown trout preovulatory follicles treated with rtTNFα in vitro. Preovulatory trout follicles were incubated in the absence or presence of coho salmon LH (sLH; 25 ng/ml), different concentrations of rtTNFα (1, 10 and 50 ng/ml) (A) or sLH in the absence or presence of 50 ng/ml of rtTNFα (B) for 24 h at 15°C. Testosterone levels were measured in the medium at the end of the incubation period. Each bar represents the mean ± SE of two independent experiments, each with ovarian tissue from a separate female and assayed in triplicate. Statistically significant (p < 0.05) differences among groups are indicated by different letters.

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