Figure 3From: Cellular and molecular evidence for a role of tumor necrosis factor alpha in the ovulatory mechanism of troutEffects of rtTNFα treatment in vitro on follicle contraction in brown trout preovulatory follicles. Preovulatory brown trout ovarian follicles were incubated for 16 h at 15°C in the presence or absence of the test compounds epinephrine and rtTNFα. Each bar represents the mean ± SE from three independent experiments, each performed with preovulatory follicles from a different female and assayed in triplicate. The results are expressed as percent change with respect to the unpunctured control group (UP control) which has been set at 100%. Punctured (P) trout preovulatory ovarian follicles were incubated in the absence or presence of epinephrine (10 μM) or rtTNFα (50 ng/ml). Statistically significant (p < 0.05) differences among groups are indicated by different letters.Back to article page