Figure 4

In situ hybridization analysis of caspase-1 mRNA expression in porcine endometrium. Protected transcripts in endometrium from day 15 of pregnancy was visualized by liquid emulsion autoradiography and imaged under bright-field and dark-field illumination. Endometrial sections from E treated section on Day 12 of pregnancy was hybridized with radiolabeled sense cRNA probe to serve as a negative control. Caspase-1 mRNA expression is abundant in the luminal (L) and glandular (G) epithelium but low to absent in the stroma (S). 4× Objective and 10× eyepiece.