Figure 3

Apoptotic population and flow cytometric analysis of granulosa cell cycle distribution. (A) Flow cytometric analysis of calcium phosphate nanoparticles induced apoptosis in granulosa cells using annexin V-FITC/PI. The x-axis represents fluorescent intensity on a logarithmic scale, whereas the y-axis represents the number of events: (A1) Control; (A2) Treatment with 10 μM calcium phosphate nanoparticles; (A3) Treatment with 100 μM calcium phosphate nanoparticles; the green peak refers to the apoptotic population. (B C) The results were analyzed by Mod Fit LT 3.0; Data were presented as mean ± SD (n = 3). *p < 0.05, compared with the control group (0 μM).