Figure 3

Photomicrographs of testicular sections of Sham Control, Amifostine, Doxorubicin and Amifostine/Doxorubicin treated rats at different ages. PAS+H method. Portions of seminiferous tubule sections of 90-day-old rats. A and B: Sham Control (A) and Amifostine (B) groups showing normal organization of the seminiferous epithelium. C and D: Doxorubicin-treated group; in C, tubular sections with partial depletion of the seminiferous epithelium are noted; some areas of discontinuity in the germ cell layers can be observed (arrows); in D, a tubular section with accentuated cellular depletion and showing Sertolization is shown. E and F: Amifostine/Doxorubicin-treated group; in both figures, note the concentric and normally organized germ cell layers of the seminiferous epithelium; pachytene spermatocytes (thick arrows); Sertoli cell nucleus (S); round spermatids (arrowheads); elongated spermatids (long arrows); tubular lumen (TL).