Figure 7
Effect of rh adiponectin on 24-h nuclear maturation of bovine oocyte in vitro. In vitro maturation of COC was performed in TCM 199 serum-free medium (control) supplemented or not with EGF (10 ng/mL) + 10% FBS (positive control), or rh adiponectin (10 μg/mL) ± insulin (10-8 M) for 24 h. The percentage of oocytes in metaphase-II stage was determined by chromatin labelling with Hoechst33342, as described in material and methods section. The data represents mean values ± SE from three independent experiments. Bars with different superscripts are significantly different (P < 0.05).