Figure 4

Effect of rh adiponectin on steroidogenesis of bovine granulosa cells. Progesterone (A) and estradiol (B) secretions were measured by RIA protocol in culture medium of granulosa cells after 48 h of culture in enriched McCoy's 5A medium (without FBS) with or without rh adiponectin (10 μg/mL), +/- IGF-I (10^-8 M), or insulin (10^-8 M). The data are expressed as the amount of steroids (ng/mL) secreted per 48 h per 50 μg protein and per basal amount. The results, expressed as means ± SE, are representative of four independent cultures with each condition in quintuplet. Bars with different superscripts are significantly different (P < 0.05).