Figure 2

Microarray validation by quantitative real-time PCR (Q-PCR). Q-PCR were achieved for six genes randomly selected among those for which microarrays reveal a sexual difference and/or flutamide sensitivity: cyclin-dependant kinase 6 (Cdk6), nuclear factor I/B (Nfib), pre B-cell leukemia transcription factor 1 (Pbx1), phosphatase and tensin homolog (Pten), sirtuin 1 (Sirt1), and nuclear receptor subfamily 2, group C, member 2 (Nr2c2). Gene expression data are shown as a relative fold-change of the second experimental group over the first experimental group in each comparison. Genes showing increased expression in male (m) compared to female (f) or in male (m) compared to flutamide treated male (flut) are placed above the axis and those with decreased expression below the axis.