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Table 1 Quantification of polysomal loading of Ldhc and Smcp mRNAs at different stages by different methods

From: Quantitative analysis of mRNA translation in mammalian spermatogenic cells with sucrose and Nycodenz gradients

mRNA(Age)1

Nycodenz2Phos3

Nycodenz qPCR3

Sucrose2Phos

Sucrose qPCR

Immuno2Phos

LdhC (21 dpp)

33.4, 36.04

34.6 ± 3.2 (6)

57.7, 58.1

51.0 ± 9.3 (6)

5ND

LdhC (Adult)

32.6 ± 2.7 (3)

28.4

56.3 ± 2.1 (9)

56.8 ± 2.7 (5)

ND

Smcp (21 dpp)

2.2 ± 0.6 (3)

3.0, 4.3

6.7, 8.3

3.7 ± 2.8 (4)

ND

Smcp (Adult)

32.8 ± 3.2 (3)

33.8

35.3 ± 4.9 (11)

29.8 ± 9.3 (4)

ND

Prm1 (25 dpp)

ND

ND

ND

ND

9.8 ± 1.9 (3)

Prm1 (32 dpp)

ND

ND

ND

ND

29.8 ± 3.7 (3)

Prm1 (Adult)

ND

ND

24.5 ± 6.5 (3)

ND

ND

  1. 1Name of species of mRNA and (age of testes analyzed)
  2. 2Method of separation of free-mRNPs and polysomal mRNAs: sucrose gradient, Nycodenz gradient, immunoprecipitation of HA-epitope tagged RP22 [16]
  3. 3Method of quantification of levels of mRNA: phosphorimage of Northern blots or slot blots or RT-qPCR
  4. 4Percent of mRNA associated with polysomes. The results are expressed and mean and standard deviation with number of replicate determinations in parentheses. The absolute values are given when there one or two replicates. The values are derived from references [4–6, 16].
  5. 5ND, not determined
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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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