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Table 1 Primer sequences

From: Time-dependent mRNA expression of selected pro-inflammatory factors in the endometrium of primiparous cows postpartum

Gene

Primer sequence

Reference

Fragment size (bp)

Annealing temperature

CXCL5

for 5'-TGA GAC TGC TAT CCA GCC G-3'

[7]

193 bp

61°C

 

rev 5'-AGA TCA CTG ACC GTT TTG GG-3'

   

IL1B

for 5'-CAA GGA GAG GAA AGA GAC A-3'

[29]

236 bp

56°C

 

rev 5'-TGA GAA GTG CTG ATG TAC CA-3'

   

IL6

for 5'-TCC AGA ACG AGT ATG AGG-3'

[29]

236 bp

56°C

 

rev 5'-CAT CCG AAT AGC TCT CAG-3'

   

IL8

for 5'-CGA TGC CAA TGC ATA AAA AC-3'

[7]

153 bp

56°C

 

rev 5'-CTT TTC CTT GGG GTT TAG GC-3'

   

PTGS2

for 5'-CTC TTC CTC CTG TGC CTG AT-3'

[11]

359 bp

60°C

 

rev 5'-CTG AGT ATC TTT GAC TGT GGG AG-3'

   

HP

for 5'-TGG TCT CCC AGC ATA ACC TC-3'

[7]

217 bp

60°C

 

rev 5'-TTG ATG AGC CCA ATG TCT ACC-3'

   

TNF

for 5'-CAA GTA ACA AGC CGG TAG CC-3'

[30]

354 bp

60°C

 

rev 5'-GCT GGA AGA CTC CTC CCT G-3'

   

18S rRNA

for 5'-GAG AAA CGG CTA CCA CAT CCA A-3'

[11]

337 bp

61°C

 

rev 5'-GAC ACT CAG CTA AGA GCA TCG A-3'

   
  1. Gene transcripts, primer sequences and annealing temperatures used for real-time PCR with resulting amplicon length.