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Figure 2 | Reproductive Biology and Endocrinology

Figure 2

From: Age-dependent role of steroids in the regulation of growth of the hen follicular wall

Figure 2

Reproductive parameters in YLC hens injected with LH and/or AG. The hens were subcutaneously injected with saline or 100 mg AG at 9.5 and 11.5 h after ovulation. Immediately after the second treatment with AG, the hens were intravenously injected with saline or 100 μg oLH. The hens were sacrificed at 3.5 h after the treatment with AG or oLH, corresponding to 15 h after ovulation. The schedule of the treatments is given in Table 1. Numbers of hens: n = 9 (Control and AG), n = 7 (LH) and n = 8 (LH+AG). Results are expressed as means ± SEM. A) The total volume of pre-ovulatory follicles (F1-F5). Means with different letters differ significantly (at least P < 0.05). B) Plasma concentrations of estradiol-17β (E2), testosterone (T) and progesterone (P4). Means for each hormone with different letters differ significantly (at least P < 0.05).

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