Table 1 Oligonucleotide primers used for PCR amplification of cDNAs obtained after reverse transcription from human testis
From: Expression of the TGF-beta1 system in human testicular pathologies
Target (Accession number) | Sequence of primer (5'___ 3') | Tm (°C) | Amplified product (bp) |
|---|---|---|---|
TGF-β1 (NM_011577) | F: TCCTGCTTCTCATGGCCA R: CCTCAGCTGCACTTGTAG | 55 | 405 |
TGFBRII (NM_009371) | F: GCGCCAACAACATCAACC R: GGTACTCCTGCAGGTTGC | 56 | 300 |
ALK-5 (NM_004612) | F: GACATCCCTGGTCCATCC R: TCTGGCACAACTCCACTG | 55 | 299 |
ALK-1 (NM_000020) | F: GTCCTGGTTCCGGGAGA R: TTGCTCTTGACCAGCACA | 55 | 294 |
Endoglin (NM_000118) | F: GCATCCTTCGTGGAGCTA R: GACACCTGCATGCCACA | 55 | 300 |
β- Actin (AJ312092) | F: GGATGCAGAAGGAGATCA R: CTAGAAGCATTTGCGGTG | 55 | 193 |