Figure 5

CD133 expression is regulated by methylation in human endometrial cancer cell lines. CD133 expression was assessed in triplicate by RT-PCR in the Hec1A, Hec1B, AN3CA and Ishikawa cell lines following treatment in vitro with either vehicle (0.005% DMSO, V1-V3) or 5 μM 5-aza-deoxycytidine (M1-M3). CD133 mRNA levels were increased in Hec1A, Hec1B and AN3CA cells following treatment with 5-aza-deoxycytidine. β-actin expression was used to confirm equivalent RNA concentrations in all samples.