Short term hypothyroidism affects ovarian function in the cycling rat

Table 1 Primer sequences and reaction conditions used in the PCR amplification of the various cDNAs

Gene	Sense primer (5'-3')	Anti-sense primer (5'-3')	Added cDNA (ng)	No. of cycles	Gene bank accession N°	Product size (pb)	Ref.
IGF I	AAAATCAGCAGTCTTCCAAC	AGATCACAGCTCCGGAAGCA	50	25	[GenBank:X06108]	299	[49]
S16	TCCAAGGGTCCGCTGCAGTC	CGTTCACCTTGATGAGCCCATT	100	22	[GenBank:XM_341815]	100	[50]
IGFBP3	GCCGCGGGCTCTGCGTCAACGC	CTGGGACTCAGCACATTGAGGAAC	200	25	[GenBank:NM_012588]	415	[49]
IGFBP5	TTGCCTCAACGAAAAGAGC	AGAATCCTTTGCGGTCACA	100	25	[GenBank:NM_012817]	377	[49]
GHR	GAGGAGGTGAACACCATCTTGGGC	ACCACCTGCTGGTGTAATGTC	100	25	[GenBank:J04811]	534	[50]
ERα	AATTCTGACAATCGACGCCAG	GTGCTTCAACATTCTCCCTCCTC	200	25	[GenBank:X061098]	344	[50]
ERβ	AAAGCCAAGAGAAACGGTGGGCAT	GCCAATCATGTGCACCAGTTCCT	100	25	[GenBank:U57439]	352	[50]
PRLLong	AAAGTATCTTGTCCAGACTCGCTG	AGCAGTTCTTCAGACTTGCCCTT	100	30	[GenBank:M74152]	279	[50]
IGFIR	TCCACCATAGACTGGTCTCT	ACGAAGCCATCTGAGTCACT	50	30	[GenBank:L29232]	433	[50]
P450arom	TGCACAGGCTCGAGTATTTCC	ATTTCCACAATGGGGCTGTCC	100	30	[GenBank:M33986]	271	[29]