Figure 5

Effects of exogenous CRH or ACTH on expression levels of Pomc , Star , Hsd3b1 , and Cyp21a1 in mouse fetal lung explants. Fetal lungs were harvested on GD 17.5 and incubated 8h in the presence of 10^-7 M CRH or 10^-7 M ACTH. Messenger RNA levels of Pomc, Star, Hsd3b1, and Cyp21a1 were measured by QPCR in control and treated lungs. Data are presented as ratios of treated samples over control samples ± SEM. Experiments were performed with 1 to 3 fetal lungs per condition per litter (n = 4 litters), and statistical analysis was performed using one-way ANOVA with randomized-block design (samples within each litter were matched). *, P < 0.05