Figure 7
From: Adiponectin promotes syncytialisation of BeWo cell line and primary trophoblast cells
Role of PKA pathway in differentiating effects of adiponectin in BeWo cells. BeWo cells were exposed to adiponectin (250 ng/ml) or PKA inhibitor H89 (10 μM) or adiponectin with H89 or foskolin alone (50 μM) for 48 h. A. Effects of adiponectin on cAMP production by BeWo cells was measured in the culture medium as described in the Material and Methods section. The values are the mean ± SEM obtained from 9-10 separate experiments. B. Effect of H89 on adiponectin hCG production by BeWo cells. hCG secretion was measured in the culture medium as described in Material and Methods section. The values are the mean ± SEM obtained from 7-8 separate experiments and expressed as fold-over the control value (untreated). *: p < 0.05; ***: p < 0.001; ns : non significant. (a): vs control (without H89 nor adiponectin). (b): adiponectin + H89 vs H89 alone.