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Table 1 Summary of real-time PCR primers

From: Spatiotemporal expression of the serine protease inhibitor, SERPINE2, in the mouse placenta and uterus during the estrous cycle, pregnancy, and lactation

Genea Primer Sequence Position Product size (bp)
Serpina5     
  Fb 5'-TCTCCATTGAGGCTACCTACAAACT-3' 1071-1095  
  Rc 5'-GTGCACCATCTCAGACAACTTGA-3' 1201-1179 131
Serpinb2     
  F 5'-TTCCGTGTGAACTCGCATGA-3' 664-683  
  R 5'-GGAAGCAACAGGAGCATGCT-3' 806-787 143
Serpine1     
  F 5'-CAGAGCAACAAGTTCAACTACACTGA-3' 810-835  
  R 5'-CAGCGATGAACATGCTGAGG-3' 915-896 106
Serpine2     
  F 5'-CAGATCATCAAGTCACGGCCT-3' 269-289  
  R 5'-ACCGTGGAGAGCTGCTTCTTT-3' 387-367 119
Plat     
  F 5'-AAGAGAGCAGCTCTGTTGGCAC-3' 1366-1387  
  R 5'-AATGGAGACGATGCCTCATGC-3' 1477-1457 112
Plau     
  F 5'-GAAGCGACCCTGGTGCTATG-3' 445-464  
  R 5'-TTTGCTAAGAGAGCAGTCATGCA-3' 526-504 82
Hprt     
  F 5'-GAATCACGTTTGTGTCATTAGTGAAA-3' 752-777  
  R 5'-TGCGCTCATCTTAGGCTTTGTA-3' 813-792 62
  1. a GenBank accession nos.: Serpina5, NM_172953; Serpinb2, NM_011111; Serpine1, NM_008871; Serpine2, NM_009255; Plat, NM_008872; Plau, NM_008873; Hprt, NM_013556
  2. b F, forward primer
  3. c R, reverse primer
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Reproductive Biology and Endocrinology

ISSN: 1477-7827

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