Figure 7
From: Transforming growth factor beta family expression at the bovine feto-maternal interface
Immunological activin βA analysis in trophoblast cells (BT-1). (a) Activin protein was detected by Western blotting using anti-activin βA sera. Lane 1: purified activin as a positive control, lane 2: culture medium of BT-1 cells, lane 3: BT-1 cell extract.
(b) Activin protein was detected using anti-activin βA sera, which was absorbed with an excess of purified activin βA protein (Lane 3, 4). Lanes 1 and 3: purified activin A, lanes 2 and 4: BT-1 extracts.